%0 Journal Article
%T Follicular fluid and supernatant from cultured cumulus-granulosa cells improve inˋvitro maturation in patients with polycystic ovarian syndrome - Fertility and Sterility
%A Aicha Madkour
%A Brahim Saadani
%A Henri Copin
%A Ismail Kaarouch
%A Moncef Benkhalifa
%A Noureddine Louanjli
%A Nouzha Bouamoud
%A Omar Sefrioui
%A Saaid Amzazi
%A Said Assou
%A Smahane Aboulmaouahib
%J Fertility and Sterility
%D 2018
%R https://doi.org/10.1016/j.fertnstert.2018.04.038
%X To study the effectiveness of a new in vitro maturation (IVM) approach based on heterologous follicular fluid (HFF) and supernatant of cumulus-granulosa cells (CGCs) mimicking the intact follicular microenvironment to rescue immature denuded oocytes (IDOs) of patients with polycystic ovary syndrome (PCOS) whose IVM or IVF outcomes remain poor. Randomized controlled trial. University-affiliated private center. One hundred fifty-nine IDOs were obtained from 47 patients with PCOS. First, a simple IVM system (S-IVM; 40 IDOs; control group) was compared with different protocols based on the addition of autologous follicular fluid (AFF-IVM; 44 IDOs), HFF (HFF-IVM; 42 IDO), or HFF with CGC isolated from seven women without PCOS and presenting 100% in vivo oocyte maturation (HFF/CGC-IVM; 33 IDOs). None. IVM outcomes were compared among the four groups (S-IVM, AFF-IVM, HFF-IVM, HFF/CGC-IVM); then the vitro and in vivo maturation results (from controlled ovarian stimulation of PCOS patients) were compared for each group. The HFF/CGC-IVM method gave the best yield of developed blastocysts per IDO compared with S-IVM, AFF-IVM, and HFF-IVM (27% vs. 2%, 2%, and 12%, respectively). The IVM rate with the HFF/CGC-IVM method was even higher than that compared with the in vivo maturation rate (79% vs. 42%), with significant improvement in the cleavage rate (71% vs. 61%). This adapted IVM system could be used to reach an acceptable result in meiotic competence and competent metaphase II oocytes capable of developing into intact embryos after fertilization and before transfer. El l赤quido folicular y el sobrenadante de cultivos de c谷lulas del c迆mulo-granulosa mejoran la maduraci車n in vitro en pacientes con s赤ndrome de ovario poliqu赤stico. Estudiar la efectividad de un nuevo abordaje de maduraci車n in vitro (IVM) basado en l赤quido folicular heter車logo (HFF) y sobrenandante de cultivo de cumulus-c谷lulas de la granulosa (CGCs) imitando el microambiente folicular intacto para rescatar ovocitos inmaduros denudados (IDOs) de pacientes con s赤ndrome de ovario poliqu赤stico (PCOS) cuyos resultados en IVM o IVF son pobres. Estudio aleatorizado controlado. Centro privado afiliado universidad. Se obtuvieron 159 IDOs de 47 pacientes con PCOS. Primero, un sistema de IVM simple (grupo control: S-IVM; 40 IDOs) se compar車 con diferentes protocolos basados en la adici車n de l赤quido folicular aut車logo (AFF-IVM; 44 IDOs), HHF (HHF-IVM; 42 IDO), o HFF con CGC aislado de siete mujeres sin PCOS y que presentaron una maduraci車n ovocitaria in vivo del 100% (HFF/CGC-IVM; 33 IDOs). ninguna. Los
%U https://www.fertstert.org/article/S0015-0282(18)30392-3/fulltext