%0 Journal Article
%T GENETIC DIVERSITY & TRANSFERABILITY OF CHICKPEA - SEQUENCE TAGGED MICROSATELLITE SITES (STMS) PRIMERS TO VIGNA & PHASEOLUS SPECIES
%A ARORA
%A A.
%A BHARETI
%A P.
%A PANWAR
%A R.K.
%A VERMA
%A S.K.
%J BioInfo Publication
%D 2019
%X Eight chickpea STMS primers were used to amplify 31 genotypes of eight Vigna & one Phaseolus species collected from GBPUA & T, Pantnagar, PCPGR, Pantnagar & Banaras Hindu University Banaras. Number of alleles per locus ranged from 3 for STMS primer TA59, which varied in size from 100 to 500 bp. Amplification of genomic DNA of thirty one genotypes, yielded 10 fragments, with an overall mean of 0.8 alleles per locus of which 9 were polymorphic while the remaining one was monomorphic. The eight STMS primers show an overall of 95.833% polymorphism. The PIC value ranged from 0.54, for locus TA59, to 0.375, for locus TA27, with a mean of 0.29. The cluster analysis using the UPGMA method displayed three clusters with 2, 5 & 24 genotypes respectively. The locus-wise total gene diversity (HT) ranged from 0.177 at TA59 to 0.499 at TA27, with an overall mean of 0.354 & the major allele frequency ranged from 0.516 for TA27 to 0.8925 for TA59, with an overall mean of 0.740. Five out of the eight chickpea STMS primer-pairs were >70% successfully transferable across the Vigna & Phaseolus species. The highest level of successful amplifications with a single primer TA110 was 83.87%. The present study indicates that the locus-specificity, co-dominant nature & transferability of STMS markers permit the fast & high throughout fingerprinting of genotypes from one genus to other to estimate their genetic diversity
%U https://bioinfopublication.org/viewhtml.php?artid=BIA0004972