%0 Journal Article
%T Absence of endothelial ¦Á5¦Â1 integrin triggers early onset of experimental autoimmune encephalomyelitis due to reduced vascular remodeling and compromised vascular integrity
%A Gregory J. Bix
%A Ravi Kant
%A Sebok K. Halder
%J Archive of "Acta Neuropathologica Communications".
%D 2019
%R 10.1186/s40478-019-0659-9
%X Upregulated expression of fibronectin and ¦Á5 integrin on blood vessels in the lumbar spinal cord during EAE. a. Time-course of increasing EAE severity following immunization. Points represent the mean£¿¡À£¿SD (n£¿=£¿10 mice). b and c. Quantification of fibronectin (b) or ¦Á5 integrin (c) fluorescent signal at different time-points of EAE progression. Results are expressed as the mean£¿¡À£¿SEM (n£¿=£¿4 mice/group). d and e. Representative examples of fibronectin and ¦Á5 integrin staining. Dual-IF was performed on frozen sections of lumbar spinal cord taken from mice that were disease-free (D-F), or in the pre-symptomatic (Pre-sym) or peak symptomatic (Symp) phase of EAE using antibodies specific for CD31 (AlexaFluor-488) and fibronectin (Fn) (Cy-3) in panel d or for CD31 (AlexaFluor-488) and ¦Á5 integrin (Cy-3) in panel e. Scale bar£¿=£¿100£¿¦Ìm. Note that in the pre-symptomatic phase of EAE, vascular expression of both fibronectin and ¦Á5 integrin was significantly increased, and this enhanced expression level was maintained during the symptomatic phase of disease. * p£¿<£¿0.05 vs. disease-free contro
%K Endothelial
%K Extracellular matrix
%K Fibronectin
%K Integrin
%K Experimental autoimmune encephalomyelitis
%K Blood-brain barrier
%K Vascular
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346510/