%0 Journal Article
%T AQP4ex is crucial for the anchoring of AQP4 at the astrocyte end-feet and for neuromyelitis optica antibody binding
%A Antonio Frigeri
%A Cinzia Buccoliero
%A Claudia Palazzo
%A Grazia Paola Nicchia
%A Maria Grazia Mola
%A Maria Trojano
%A Pasqua Abbrescia
%J Archive of "Acta Neuropathologica Communications".
%D 2019
%R 10.1186/s40478-019-0707-5
%X Generation of the AQP4ex £¿/£¿ mouse. a Schematic depiction of the targeting strategy. The mouse Aqp4 gene contains five exons (vertical bars). Exon 5 contains the target site for the knockin TAATAGTGA sequence. After Cas9-mediated DNA cleavage, the knockin TAATAGTGA sequence was introduced into Exon 5 by homology-directed repair. b Mouse AQP4ex-KO generation. gRNA obtained by in vitro transcription and donor oligo were co-injected into fertilized eggs for KI mouse production. c DNA sequencing analysis. The 617£¿bp long PCR product was purified and sequenced to analyze the DNA sequence in the target site (highlighted in red) of wild-type (WT) heterozygous (HET) and KO mice. d. DNA restriction enzyme analysis. Mae III fragments obtained by complete digestion of KO, lane 1 (125, 220, 272£¿bp) wildtype, line 2 (226£¿bp and 391£¿bp) and heterozygous, line 3 and 4 (125, 220, 226, 272 and 391£¿bp) PCR products. The PCR product without MAE III enzyme digestion is shown in line
%K Aquaporin-4
%K Astrocyte endfoot
%K Blood brain barrier
%K Orthogonal arrays of particles
%K OAPs
%K Translational readthrough
%K Neuromyelitis optica
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444679/