%0 Journal Article
%T Extraction of Honey Polyphenols: Method Development and Evidence of Cis Isomerization
%A Aurore Richel
%A Bach Kim Nguyen
%A Eric Haubruge
%A Nicolas Jacquet
%A Thibaut Istasse
%A Thomas Berchem
%J Archive of "Analytical Chemistry Insights".
%D 2016
%R 10.4137/ACI.S39739
%X Honey polyphenols have been studied with the objective of relating honeys to their floral sources. Initially synthesized by plant, these polyphenols can be found in the plant¡¯s nectar, which are collected by bees, which convert the nectar into honey. Consequently, polyphenols constitute minor components of honey. The development of a solid-phase extraction method for honey polyphenols is presented in this study. The technique employs Amberlite XAD-2 adsorbent and was tested on monofloral honeys from six different plants: acacia, chestnut, eucalyptus, thyme, sunflower, and wild carrot. Analyses were performed using high-performance liquid chromatography coupled with UV detection and mass spectrometry. Several phenolic acids and flavonoids were identified: caffeic and p-coumaric acids, quercetin, kaempferol, naringenin, chrysin, and pinocembrin. Generally, the quantity of a given polyphenol in the honey was around 0.2 mg/100 g of honey, except for chestnut honey, which contained around 3.0 mg of p-coumaric acid/100 g of honey. Analyses highlighted significant formation of cis isomers for phenolic acids during the extraction despite protection from light
%K polyphenols
%K honey
%K solid-phase extraction
%K isomerization
%K chromatography
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4981221/