%0 Journal Article
%T Stability-Indicating High-Performance Liquid Chromatography Assay for the Determination of Sulthiame in Pharmaceutical Dosage Forms
%A Ammar Haidar
%A Elyes Majoul
%A Issa-Bella Balde
%A Jean-Eudes Fontan
%A Jo£¿l Schlatter
%A Salvatore Cisternino
%A Sofiane Kabiche
%J Archive of "Analytical Chemistry Insights".
%D 2016
%R 10.4137/ACI.S38656
%X A stability-indicating assay by reversed-phase high performance liquid chromatography method was developed and validated for the determination of sulthiame (STM). The chromatographic separation was achieved on a reversed-phase NovaPack C18 column and an isocratic mobile phase consisting of deionized water:methanol (70:30, v/v). The flow rate was 1.0 mL/min (ultraviolet detection at 210 nm). The STM was separated within 2.83 min. The linearity of the method was demonstrated in the range of 20.0¨C200.0 ¦Ìg/mL and a coefficient of determination of r2 = 0.9999. The limits of detection and quantification were 4.2 and 9.5 ¦Ìg/mL, respectively. The intraday and interday precisions were less than 1%. Accuracy of the method ranged from 98.3% to 101.7%, with a relative standard deviation of <1%. STM was degraded by accelerated breakdown in alkaline, acidic, or oxidative stress conditions. This method allows accurate and reliable determination of STM for drug stability assay in pharmaceutical studies
%K sulthiame
%K liquid chromatography
%K stability indicating
%K forced degradation
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013865/