%0 Journal Article
%T Development of Hollow-Fiber Liquid-Phase Microextraction Method for Determination of Urinary trans,trans-Muconic Acid as a Biomarker of Benzene Exposure
%A Abbas Moghimbeigi
%A Abdulrahman Bahrami
%A Farhad Ghamari
%A Farshid Ghorbani Shahna
%A Yadollah Yamini
%J Archive of "Analytical Chemistry Insights".
%D 2016
%R 10.4137/ACI.S40177
%X For the first time, hollow-fiber liquid-phase microextraction combined with high-performance liquid chromatography¨Cultraviolet was used to extract trans,trans-muconic acid, in urine samples of workers who had been exposed to benzene. The parameters affecting the metabolite extraction were optimized as follows: the volume of sample solution was 11 mL with pH 2, liquid membrane containing dihexyl ether as the supporter, 15% (w/v) of trioctylphosphine oxide as the carrier, the time of extraction was 120 minutes, and stirring rate was 500 rpm. Organic phase impregnated in the pores of a hollow fiber was extracted into 24 ¦ÌL solution of 0.05 mol L£¿1 Na2CO3 located inside the lumen of the fiber. Under optimized conditions, a high enrichment factor of 153¨C182 folds, relative recovery of 83%¨C92%, and detection limit of 0.001 ¦Ìg mL£¿1 were obtained. The method was successfully applied to the analysis of ttMA in real urine samples
%K trans
%K trans-muconic acid
%K benzene exposure
%K carrier-mediated hollow-fiber liquid-phase microextraction
%K HPLC-UV
%K human urine
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019127/