%0 Journal Article
%T lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs
%A Jia-Yu Zhong
%A Jie-Yu He
%A Jun-Kun Zhan
%A Pan Tan
%A Shuang Li
%A Xiao Lin
%A Xing-Jun Cui
%A Xue-Bin Liu
%A Yan-Jiao Wang
%A Yi Wang
%A Yi-Yin Chen
%A You-Shuo Liu
%J Archive of "Aging (Albany NY)".
%D 2019
%R 10.18632/aging.101758
%X Vascular calcification/aging is common in diabetes and is associated with increased morbidity and mortality of patients. MiR-34c-5p, not miR-34c-3p, was suppressed significantly in calcification/senescence of human aorta vascular smooth muscle cells (HA-VSMCs) induced by high glucose, which was proven by the formation of mineralized nodules and staining of senescence associated-¦Â-galactosidase staining (SA ¦Â-gal) positive cells. Overexpression of miR-34c-5p alleviated calcification/senescence of HA-VSMCs, whereas inhibition of miR-34c-5p received the opposite results. Bcl-2 modifying factor (BMF) was a functional target of miR-34c-5p and it was involved in the process of calcification/senescence of HA-VSMCs. Besides, lncRNA-ES3 acted as a competing endogenous RNAs (ceRNA) of miR-34c-5p to enhance BMF expression. Further, lncRNA-ES3 inhibited miR-34c-5p expression by direct interaction and its knockdown suppressed the calcification/senescence of HA-VSMCs. Our results showed for the first time that the calcification/senescence of VSMCs was regulated by lncRNA-ES3 /miR-34c-5p/BMF axis
%K miR-34c-5p
%K lncRNA-ES3
%K BMF
%K VSMCs calcification/senescence
%K vascular aging
%K diabetes
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366973/