%0 Journal Article
%T Iatrogenic early onset cerebral amyloid angiopathy 30£¿years after cerebral trauma with neurosurgery: vascular amyloid deposits are made up of both A¦Â40 and A¦Â42
%A Alessandra Erbetta
%A Anna Bersano
%A Antonio Indaco
%A Emanuela Maderna
%A Eugenio Parati
%A Gianluca Marucci
%A Giorgio Giaccone
%A Giuseppe Di Fede
%A Luigi Caputi
%A Luisa Chiapparini
%A Marcella Catania
%A Paola Caroppo
%J Archive of "Acta Neuropathologica Communications".
%D 2019
%R 10.1186/s40478-019-0719-1
%X Neuropathologic findings of the cerebral biopsy. Severe amyloid angiopathy appeared as thickening of the wall of parenchymal arterioles (a, Haematoxylin &Eosin) where amorphous material, fluorescent after thioflavine S treatment, built up (b, thioflavine S). When antibody 4G8 was used (mouse monoclonal, 1:2000, after 80% formic acid for 20£¿min) that recognizes the different A¦Â species (epitope at residues 17¨C24 of A¦Â), immunoreactivity was intense both in parenchymal (c) and leptomeningeal vessels (d). Both the antibody specific for A¦Â40 (mouse monoclonal, Covance, 1:1000, after 80% formic acid for 20£¿min)(e) and that specific for A¦Â42 (mouse monoclonal, Covance, 1:500, after 80% formic acid for 20£¿min)(f) strongly decorated the amyloid-laden vessels. Compact A¦Â deposits were present in the neuropil (g, thioflavine S) and were intensely immunolabeled by anti-A¦Â42 (not shown) and 4G8 (h), while tau pathology was minimal, appearing as cellular profiles immunopositive for anti-phosphorylated tau antibody AT8 (mouse monoclonal, Biosource, 1:300) often surrounding amyloid laden vessels (i). Immunolabeling was visualized by the Envision Plus/Horseradish Peroxidase System (DakoCytomation) using 3¨C3¡ä-diaminobenzidine (brown reaction product) as chromogen. Bar in A£¿=£¿25£¿¦Ìm (A,B,G,H and I are the same magnification); bar in C£¿=£¿100£¿¦Ìm (C,D,E and F are the same magnification
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498603/