%0 Journal Article
%T Optimizing peptide epitope-based autoantibody detection in cancer patients
%A Allan J Pantuck
%A Gang Zeng
%A Maize Wang
%A Sarah Lee
%A Shirley H Lomeli
%A Wilbur A Franklin
%J Archive of "American Journal of Clinical and Experimental Immunology".
%D 2017
%X Autoantibody (autoAb) response is an important arm of endogenously arising anti-tumor immune responses, and has received new attention as a cancer biomarker with the recent success of immune check-point inhibitor therapy. Our laboratory has been focusing on measuring autoAb against B-cell epitopes in order to bypass the necessity to purify a panel of recombinant proteins. In order to optimize peptide-based autoAb measurement and to increase sensitivities to cover more patients, we developed a new approach of using mixed peptides to conjugate on the same microsphere and compared its results with the use of a dominant peptide epitope using Luminex microbead-based multiplex assays. The peptide epitopes of two cancer/germline antigens, New York esophageal cancer antigen-1 (NY-ESO-1) and X antigen family member-1b (XAGE-1b), and cancer/stem cell antigen, sex determining region Y-box-2 (SOX2), were used as prototypes in this study. Our results indicate that using mixed peptides of B-cell epitopes improves the sensitivity of detecting more patients with autoAb responses. Thus, when the full-length protein is not available for conjugating onto microspheres, a mixture of B-cell epitopes is the method of choice for using Luminex multiplex assay to detect autoAb response in cancer patients
%K Tumor-associated antigen
%K autoantibody
%K biomarker
%K immune monitoring
%K B cell epitope
%K multiplex assay
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5698562/