%0 Journal Article
%T Optimization and Comparison of Different Methods and Factors for Efficient Transformation of Brucella abortus RB51strain
%A Azam Gheibi
%A Bahram Kazemi
%A Gholam Ali Kardar
%A Hossein Khanahmad
%A Maryam Boshtam
%A Mohammad Reza Khorramizadeh
%A Sassan Rezaie
%J Archive of "Advanced Biomedical Research".
%D 2019
%R 10.4103/abr.abr_14_19
%X The development of protective vaccines for Brucella spp. has been hampered by the difficulty in transformation of Brucella cells with foreign DNA for genetic manipulation. It seems that the formation of Brucella spheroplasts would increase the efficiency of transformation. The aim of this study was to devise an efficient method for the transformation of Brucella spp
%K Ampicillin
%K Brucella
%K lipofectamine 2000
%K lipofection
%K spheroplasts
%K sucrose
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555225/