%0 Journal Article
%T A Device for Ribbon Collection for Array Tomography with Scanning Electron Microscopy
%A Akira Saito
%A Hisao Yamada
%A Mitsuo Suga
%A Mitsuyo Maeda
%A Taro Koike
%A Yosky Kataoka
%A Yuji Hasebe
%A Yuuki Yamaguchi
%J Archive of "Acta Histochemica et Cytochemica".
%D 2017
%R 10.1267/ahc.17013
%X ˇ°Array tomographyˇ± is a method used to observe the fine structure of cells and tissues in a three-dimensional view. In this method, serial ultrathin sections in the ribbon state (ribbons) are mounted on a solid substrate and observed by scanning electron microscopy (SEM). The method may also be used in conjunction with post-embedding immunocytochemistry. However, it is difficult to mount many serial ribbons on a substrate manually. We developed an inexpensive laboratory-made device that mounts ribbons by pulling a nylon fishing line and lifting the substrate up from the water in a knife boat. Using this device, we succeeded in mounting several ribbons consisting a mean of 205.6 (SD: 37.7) serial ultrathin sections on 1.25 (SD: 0.06) ˇÁ 1.25 (SD: 0.06)-cm silicon substrates. Furthermore, it was confirmed that our method is suitable for ribbons derived from water-soluble resin blocks. We were also able to stain the specimens by post-embedding immunocytochemistry. Thus, our method is useful in mounting numerus sections on a substrate for array tomography with SEM
%K array tomography
%K three-dimensional observation
%K serial ultrathin section
%K post-embedding labeling
%K scanning electron microscopy
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736830/