%0 Journal Article
%T Intranuclear Mobility of Estrogen Receptor: Implication for Transcriptional Regulation
%A Ken Ichi Matsuda
%A Mitsuhiro Kawata
%A Takashi Hashimoto
%J Archive of "Acta Histochemica et Cytochemica".
%D 2018
%R 10.1267/ahc.18023
%X The estrogen receptor (ER) is a ligand-dependent transcription factor that has two subtypes: ER¦Á and ER¦Â. ERs regulate transcription of estrogen-responsive genes through interactions with multiple intranuclear components, such as cofactors and the nuclear matrix. Live cell imaging using fluorescent protein-labeled ERs has revealed that ligand-activated ERs are highly mobile in the nucleus, with transient association with the DNA and nuclear matrix. Scaffold attachment factor B (SAFB) 1 and its paralogue, SAFB2, are nuclear matrix-binding proteins that negatively modulate ER¦Á-mediated transcription. Expression of SAFB1 and SAFB2 reduces the mobility of ER¦Á in the presence of ligand. This regulatory machinery is emerging as an epigenetic-like mechanism that alters transcriptional activity through control of intranuclear molecular mobility
%K estrogen receptor
%K living imaging
%K FRAP
%K SAFB
%K nuclear matrix
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160615/