%0 Journal Article
%T Competitive Luminex immunoassays for detection of antibodies to foot-and-mouth disease and vesicular stomatitis viruses in multiple susceptible hosts
%A Charles Nfon
%A Diana Lusansky
%A Kate Hole
%A Leanne McIntyre
%A Melissa Goolia
%A Ming Yang
%J Archive of "Canadian Journal of Veterinary Research".
%D 2018
%X Foot-and-mouth disease (FMD) and vesicular stomatitis (VS) cause such similar clinical signs and lesions that laboratory tests are required to distinguish between infections caused by each virus. Using mouse anti-foot-and-mouth disease virus (FMDV) 3B monoclonal or polyclonal anti-vesicular stomatitis virus-New Jersey (VSV-NJ) antibodies and recombinant FMDV 3ABC or VSV-NJ glycoprotein (G) antigens coated to MagPlex beads, competitive Luminex immunoassays (cLIAs) were developed for FMDV and VSV-NJ, respectively. The cLIAs successfully detected antibodies to FMDV 3ABC and VSV-NJ G in sera from infected animals. The diagnostic sensitivity and specificity were 93% and 98%, respectively for FMDV and 93% and 95.4%, respectively for VSV-NJ. These cLIAs are potential alternatives for competitive enzyme-linked immunosorbent assays (cELISAs) and provide the opportunity for multiplexing to reduce time and the amount of serum required for testing
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168021/