%0 Journal Article
%T Construction of a GLUT-1 and HIF-1¦Á gene knockout cell model in HEp-2 cells using the CRISPR/Cas9 technique
%A Jun Fan
%A Li-Fang Shen
%A Min-Li Zhou
%A Qi Yu
%A Shui-Hong Zhou
%A Ting-Ting Wu
%A Ya-Ping Huang
%A Yang-Yang Bao
%A Zhong-Jie Lu
%J Archive of "Cancer Management and Research".
%D 2019
%R 10.2147/CMAR.S183859
%X Glucose transporter (GLUT)-mediated glucose uptake is an important process in the development of laryngeal carcinoma, one of the most common malignancies of the head and neck. GLUT-1, together with HIF-1¦Á, is also an indicator of hypoxia. Both proteins play a critical role in glucose uptake and glycolysis in laryngeal carcinoma cells under hypoxic stress. A double gene knockout model in which HIF-1¦Á and GLUT-1 are no longer expressed can provide important information about carcinogenesis in laryngeal carcinoma
%K CRISPR
%K Cas9 system
%K glucose transporter-1
%K HEp-2 cells
%K hypoxia-inducible factor-1¦Á
%K PI3K
%K AKT
%K mTOR pathway
%K laryngeal carcinoma
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413817/