%0 Journal Article
%T Oncogenic functions of protein kinase D2 and D3 in regulating multiple cancerŠ\related pathways in breast cancer
%A Aihua Shi
%A Feifei Wang
%A Huilin Zheng
%A Jian Li
%A Jilinlin Wang
%A Jingzhong Zhang
%A Jun Zhang
%A Li An
%A Liming Chen
%A Qian Su
%A Qingfei Chen
%A Shuang Yu
%A Weiyong Zhao
%A Xinxing Ma
%A Xue Lin
%A Yan Liu
%A Yehui Zhou
%A Yiwen Yang
%A Yunchao Zhang
%A Yuzhi Wang
%A Zhenghong Yu
%A Zhi Xu
%A Zhifang Ma
%J Archive of "Cancer Medicine".
%D 2019
%R 10.1002/cam4.1938
%X Protein Kinase D (PKD) family contains PKD1, PKD2, and PKD3 in human. Compared to consistent tumorŠ\suppressive functions of PKD1 in breast cancer, how PKD2/3 functions in breast cancer are not fully understood. In the current study, we found that PKD2 and PKD3 but not PKD1 were preferentially overexpressed in breast cancer and involved in regulating cell proliferation and metastasis. Integrated phosphoproteome, transcriptome, and interactome showed that PKD2 was associated with multiple cancerŠ\related pathways, including adherent junction, regulation of actin cytoskeleton, and cell cycleŠ\related pathways. ELAVL1 was identified as a common hubŠ\node in networks of PKD2/3Š\regulated phosphoproteins and genes. Silencing ELAVL1 inhibited breast cancer growth in vitro and in vivo. Direct interaction between ELAVL1 and PKD2 or PKD3 was demonstrated. Suppression of PKD2 led to ELAVL1 translocation from the cytoplasm to the nucleus without significant affecting ELAVL1 expression. Taken together, we characterized the oncogenic functions of PKD2/3 in breast cancer and their association with cancerŠ\related pathways, which shed lights on the oncogenic roles and mechanisms of PKDs in breast cancer
%K breast cancer
%K phosphoproteome analysis
%K PKD2
%K PKD3
%K transcriptome analysis
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504119/