%0 Journal Article
%T Mixed-lineage leukemia protein 2 suppresses ciliary assembly by the modulation of actin dynamics and vesicle transport
%A Dengwen Li
%A Huijie Hao
%A Jie Ran
%A Jun Zhou
%A Min Liu
%A Song Guo
%A Te Li
%A Xiaofan Wu
%A Yang Liu
%A Yang Yang
%J Archive of "Cell Discovery".
%D 2019
%R 10.1038/s41421-019-0100-3
%X a Quantitative RT-PCR analysis of MLL2 mRNA expression in RPE-1 cells transfected with control or MLL2 siRNAs and serum-starved for 48£¿h. b Immunoblot analysis of H3K4me1 and ¦Â-actin levels in RPE-1 cells transfected with control (C) or MLL2 (M) siRNAs and serum-starved for 0, 12, 24, and 48£¿h. c¨Ce Immunofluorescence images (c), percentage of ciliated cells (d, n£¿=£¿200), and ciliary length (e, n£¿=£¿80) for RPE-1 cells transfected with control or MLL2 siRNAs, serum-starved for 48£¿h, and stained with antibodies against ¦Ã-tubulin (red) and acetylated ¦Á-tubulin (green), and the DNA dye DAPI (blue). Scale bar, 10£¿¦Ìm. f¨Ch Immunofluorescence images (f), percentage of ciliated cells (g, n£¿=£¿200), and ciliary length (h, n£¿=£¿50) for RPE-1 cells treated with the indicated concentrations of MTA, serum-starved for 48£¿h, and stained with antibodies against ¦Ã-tubulin (red) and acetylated ¦Á-tubulin (green), and DAPI (blue). Scale bar, 10£¿¦Ìm. i¨Ck Immunofluorescence images (i), percentage of ciliated cells (j, n£¿=£¿100), and ciliary length (k, n£¿=£¿30) for RPE-1 cells transfected with the indicated siRNAs and plasmids, followed by serum starvation and staining with acetylated ¦Á-tubulin antibodies (red) and DAPI (blue). Scale bar, 10£¿¦Ìm. **P£¿<£¿0.01; ***P£¿<£¿0.001. Error bars indicate SE
%K Cilia
%K Actin
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591415/