%0 Journal Article
%T Fisetin induces autophagy in pancreatic cancer cells via endoplasmic reticulum stress- and mitochondrial stress-dependent pathways
%A Guoping Ding
%A Liping Cao
%A Senhao Zhou
%A Shengnan Jia
%A Xiaodong Xu
%A Yan Chen
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1366-y
%X a Cell viability of PANC-1 cells was measured by CCK-8 assay. Cells were treated with fisetin (0, 25, 50, 100, 200, and 400£¿¦ÌM) for 24 and 48£¿h, respectively. The absorbance was measured at 450£¿nm. Data are presented as mean£¿¡À£¿SD; *P£¿¡Ü£¿0.05, #P£¿¡Ü£¿0.05. b Real-time cell analysis (RTCA) of pancreatic cancer PANC-1 cells. Cells were seeded in 96 well Eplates. Then cells were treated with fisetin (0¨C400£¿¦ÌM)for 72£¿h. Cell growth was monitored using the xCELLigence RTCA DP Instrument (Roche). Impedance was recorded every 15£¿min using an xCELLigence system. The times on the x-axis indicate the times after treatment. The Cell Index on the y-axis reflects a consistent, logarithmic relationship to cell number. Each point represents the mean value from three replicates with SDs. c A total of 1£¿¡Á£¿106 PANC-1-Luciferase cells were injected into the shoulders via a left subcostal incision. Control group mice were treated with DMSO via intraperitoneal injection, and treatment group mice were treated with Fisetin (300£¿mg/kg body weight i.p.) every other day. After 20 days treatment, mice imaged for bioluminescence. Data were expressed as average radiance (photons/second/square centimeter/steradian). d The differences in tumor volume between control and fisetin treatment groups at 20 days after treatment. Results are mean£¿¡À£¿SD (n£¿=£¿5), **P£¿¡Ü£¿0.01. e The expression levels of PCNA, cleaved-caspase 3 in control and fisetin treatment groups of mice were detected by immunohistochemistry. Bar scale 50£¿¦Ìm. f Western blotting analyses of Ki67, PCNA, p-H3, cleaved-caspase3, and cleaved PARP. g Apoptosis of cells with treatment. Cells were treated with fisetin (200£¿¦ÌM) for 24 and 48£¿h, followed by Annexin-V/PI staining and flow cytometry analysis. Data are presented as mean£¿¡À£¿SD; **P£¿¡Ü£¿0.01, ****P£¿¡Ü£¿0.0001, NS no significanc
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374379/