%0 Journal Article
%T Cul4a promotes zebrafish primitive erythropoiesis via upregulating scl and gata1 expression
%A Changshun Shao
%A Fan Yang
%A Huili Hu
%A Ming Shao
%A Yaoqin Gong
%A Yuanyuan Liu
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1629-7
%X a o-dianisidine staining showed depletion of erythrocytes in cul4a-morphant embryos, compared with controls. Zebrafish cul4a mRNA rescued primitive erythropoiesis in cul4a-morphant embryos. b Expression of the embryonic hemoglobin, hbbe3, was analyzed by WISH at 24£¿hpf in embryos injected with CoMO or cul4a-MO, or MO coinjected with zebrafish cul4a mRNA. Lateral views are shown with anterior to the left. c Relative mRNA level of hbbe3 was assayed by qRT-PCR in embryos injected with CoMO or cul4a-MO, or MO coinjected with cul4a mRNA at 24£¿hpf. d WISH was performed with hbbe3 probes in embryos at 24£¿hpf injected with CoMO or cul4a-MO1. e Relative mRNA level of hbbe3 was measured by qRT-PCR in embryos injected with CoMO or cul4a-MO1. f o-dianisidine staining showed decreased erythrocytes in cul4a£¿/£¿ and double knockout, but not in cul4b£¿/£¿ knockout embryos. Embryos shown are lateral views with anterior to the left. g The images of WISH with hbbe3 mRNA probes in control (cas9-tail injected), cul4a £¿/£¿, cul4b £¿/£¿, and double knockout mutants at 24£¿hpf. h Relative mRNA level of hbbe3 was assayed by qRT-PCR in control (cas9-tail injected), cul4a£¿/£¿, cul4b£¿/£¿, and double knockout mutants at 24£¿hpf. The number in the top right-hand corner indicates the phenotypic embryos/total embryos. qRT-PCR experiments were performed in triplicate. ***p£¿<£¿0.001. All scale bars represent 250£¿¦Ì
%K Embryogenesis
%K Erythropoiesis
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525236/