%0 Journal Article
%T abLIM1 constructs non-erythroid cortical actin networks to prevent mechanical tension-induced blebbing
%A Daniel M. Czajkowsky
%A Guoqing Li
%A Jiabin Wang
%A Jingli Cao
%A Sen Yang
%A Shan Huang
%A Xueliang Zhu
%A Zhifeng Shao
%J Archive of "Cell Discovery".
%D 2018
%R 10.1038/s41421-018-0040-3
%X a Expression of abLIM1 in different cells or mouse tissues. ¦Â-actin served as loading control. b, c abLIM1 was a cell cortex protein. Intact U2OS or RPE1 cells (b) or the cells treated with EDTA to acquire a spherical morphology (c) were subjected to immunostaining and confocal microscopy. A single optical section is shown for the cells in (c). ¦ÂII spectrin served as cell cortex marker. Arrows point to typical regions positive for both proteins. Nuclear DNA was visualized by DAPI. d Subcellular localization of GFP-abLIM1 in U2OS cells. Arrows indicate regions apparently positive for GFP-abLIM1, ¦ÂII spectrin, and F-actin. Note that highly expressed GFP-abLIM1 displayed colocalization with actin bundles. e Overexpressed GFP-abLIM1 colocalized with cortical actin bundles. Shown are confocal micrographs of a representative RPE1 cell. The two dimensional (2D) images were projected from all optical sections or just Paxillin-free top sections. The 3D reconstructed image is shown as the top view, accompanied with side views of the indicated positions. Paxillin signals mark the bottom side of the cell. f GFP failed to show associations with F-actin in RPE1 cells. Note that the cell is also abundant in cortical actin bundle
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056535/