%0 Journal Article
%T A novel antibody-TCR (AbTCR) platform combines Fab-based antigen recognition with gamma/delta-TCR signaling to facilitate T-cell cytotoxicity with low cytokine release
%A Bryan Zimdahl
%A Cheng Liu
%A David M. Barrett
%A Hong Liu
%A Javier F. Morales
%A Jingwei Lu
%A Lianxing Liu
%A Lucas H. Horan
%A Pengbo Zhang
%A Shon Green
%A Stephan A. Grupp
%A Vivien W. Chan
%A Yiyang Xu
%A Zhiyuan Yang
%J Archive of "Cell Discovery".
%D 2018
%R 10.1038/s41421-018-0066-6
%X a Schematic of our antibody-TCR (AbTCR) platform compared to a TCR and a second-generation CAR platform. b ET190L1-AbTCR is expressed on the surface of primary T cells following lentiviral transduction. Anti-human Fab antibody was used to detect AbTCR expression. Representative flow cytometry plots and images (40¡Á) of T cells stained with anti-human Fab antibody are shown. c AbTCR expression stabilizes the cell surface presentation of CD3£¿ on TCR¦Â negative J.RT3-T3.5 cells. d The CD3 complex co-immunoprecipitates with the AbTCR. Cell lysates and anti-FLAG immunoprecipitates from primary T cells expressing a FLAG-tagged ET190L1-AbTCR (or untransduced; Mock) were subjected to western blot analysis for CD3¦Ä, ¦Å, ¦Ã, and ¦Æ chain
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242878/