%0 Journal Article
%T Obesogenic high-fat diet heightens aerobic glycolysis through hyperactivation of oncogenic KRAS
%A Albert Z. Mao
%A Craig D. Logsdon
%A Dan Wang
%A Ellen Li
%A James L. Abbruzzese
%A Juntao Ji
%A Lianghao Hu
%A Vincent W. Yang
%A Weiqin Lu
%A Yawei Bi
%A Yongde Luo
%A Zhaoshen Li
%J Archive of "Cell Communication and Signaling : CCS".
%D 2019
%R 10.1186/s12964-019-0333-7
%X HFD hyperactivates oncogenic KRAS to elevate aerobic glycolysis. a Diagram of mouse treatment. TM, tamoxifen; CD (n£¿=£¿8), carbohydrate-rich diet; HFD (n£¿=£¿8), high-fat diet. b-c Western blot analysis and quantification of the expression levels of HKII in fElasCreERT and KrasG12D/+ with the indicated treatment. Three independent experiments from three different mice per group were performed. d-e Western blot analysis and quantification of the expression levels of LDHA in fElasCreERT and KrasG12D/+ with the indicated treatment. Three independent experiments from three different mice per group were performed. f-g RAS activity assay followed by Western blot analysis and quantification of the RAS-GTP levels in mice harboring fElasCreERT and KrasG12D/+ under both CD and HFD for 10 weeks. Total RAS was used as a loading control. Three independent experiments from three different mice per group were performed. h Representative IHC staining for p-ERK (upper panel) and Alcian blue staining for acidic mucins (lower panel) as indicated. Magnifications: 200x. (n£¿=£¿6 mice per group). i Statistical analysis of p-ERK (n£¿=£¿6) as represented in Fig. 1h (upper panel). j Statistical analysis of the Alcian Blue staining (n£¿=£¿6) as represented in Fig. 1h (lower panel). Data are mean£¿¡À£¿SD. p values are determined by the Student¡¯s t-test. *p£¿<£¿0.05; **p£¿<£¿0.01; ***p£¿<£¿0.001; ****p£¿<£¿0.000
%K KRAS
%K Pancreatic cancer
%K Glycolysis
%K High-fat diet
%K Obesity
%K COX-2
%K Hexokinase 2
%K Lactate dehydrogenase
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396546/