%0 Journal Article
%T Correction to: Low levels of pyruvate induced by a positive feedback loop protects cholangiocarcinoma cells from apoptosis
%A Bo Kong
%A Dehua Tang
%A Helmut Friess
%A Jian-lin Wu
%A Lei Wang
%A Lei Xu
%A Lixing Zhou
%A Mingming Zhang
%A Qian Zhou
%A Robert Gregory Dorfman
%A Shimin Zhao
%A Xiaoping Zou
%A Yang Li
%A Yida Pan
%A Yuming Wang
%A Yuyao Yin
%J Archive of "Cell Communication and Signaling : CCS".
%D 2019
%R 10.1186/s12964-019-0357-z
%X The changes in metabolic enzymes contribute to the low levels of pyruvate. a Cells were treated with 1£¿¦ÌM ML265 for 24£¿h and subjected to western blot. b PKM2 overexpressed cells and their counterparts were subjected to western blot. c The oxygen consumption rates (OCR) of PKM2-overexpressed cells and control cells were detected at different time points. OCR under oligomycin, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), and antimycin A/rotenone treatments, respectively. d Cells were treated with 10£¿¦ÌM Gossypol-Acetic Acid (GAA) for 24£¿h and subjected to western blot. e LDHA knockdown cells and their counterparts were subjected to western blot. f The OCR of LDHA knockdown cells and control cells were detected at different time points. g Cells were treated with 1£¿¦ÌM ML265 and 10£¿¦ÌM GAA for 24£¿h, then subjected to western blot. h Cells were treated with 1£¿¦ÌM ML265 and 10£¿¦ÌM GAA for 24£¿h, the concentrations of the metabolites in GC cells were measured by Mass spectrometry (MS) and normalized to their protein level. i The OCR of cells were detected at different time points after they treated with 1£¿¦ÌM ML265 and 10£¿¦ÌM GAA for 24£¿h. Data represent the Mean£¿¡À£¿SEM, n£¿¡Ý£¿3. *p£¿<£¿0.05, **p£¿<£¿0.01, NS not significan
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503371/