%0 Journal Article
%T Combination chemotherapy of valproic acid (VPA) and gemcitabine regulates STAT3/Bmi1 pathway to differentially potentiate the motility of pancreatic cancer cells
%A Chenggang Gao
%A Chunyou Wang
%A Hehe Li
%A Heshui Wu
%A Qiang Shen
%A Qingke Duan
%A Shihong Wu
%A Tao Yin
%A Zhengle Zhang
%J Archive of "Cell & Bioscience".
%D 2019
%R 10.1186/s13578-019-0312-0
%X Gemcitabine and VPA affect the cell viability, migration and invasion of pancreatic cancer cells. PANC-1 and Patu8988 cells were treated with 0¨C10 mM of VPA for 36 h (a) or 0¨C1000 ¦Ìm of gemcitabine for 24 h (b), and the relative survival rate was determined by the MTT assay. c After treatment with 0.5 mM or 5 mM VPA for 12 h, both cell lines were treated in combination with 5 ¦Ìm gemcitabine for 24 h, and cell viability was measured by the MTT assay. d The pancreatic cancer cell lines were treated as indicated above, and the migration and invasion abilities were measured by the Transwell migration and invasion assays, respectively. e The relative migration/invasion rate were represented motility abilities of pancreatic cancer cells, and it was calculated by counting the migrated/invasive cells in each treatment group relative to the control group. In a¨Ce, the data are shown from three independent experiments. n.s. no significance. *P£¿<£¿0.05; **P£¿<£¿0.01; ***P£¿<£¿0.001 compared with the contro
%K Pancreatic cancer
%K Gemcitabine
%K Valproic acid
%K Combined chemotherapy
%K Bmi1
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582499/