%0 Journal Article
%T Amino acid response by Halofuginone in Cancer cells triggers autophagy through proteasome degradation of mTOR
%A Alessandra Ferraresi
%A Carlo Follo
%A Chiara Vidoni
%A Christian Seca
%A Ciro Isidoro
%A Federica Morani
%J Archive of "Cell Communication and Signaling : CCS".
%D 2019
%R 10.1186/s12964-019-0354-2
%X Halofuginone induces the amino acid starvation response (AAR) pathway. Thyroid cancer WRO cells, breast cancer MCF-7 cells, and ovarian cancer OAW-42 cells were exposed to 100£¿nM halofuginone (HF) for 4, 8 and 24£¿h. Total and phosphorylated (Ser 51) eIF2¦Á protein levels were assessed by immunoblotting. As loading control, filters were stripped and probed with anti ¦Â-Tubulin as loading control. Representative immunoblots from three independent experiments are shown along with P-eIF2¦Á/eIF2¦Á band intensity ratios as index of AA
%K Autophagy
%K Starvation
%K mTORC1
%K Lysosome
%K TFEB
%K Protein translation
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498594/