%0 Journal Article
%T Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-¦ÊB pathways
%A Haijun Li
%A Hongming Lv
%A Huahong Yang
%A Liping Peng
%A Xinxin Ci
%J Archive of "Cell Communication and Signaling : CCS".
%D 2019
%R 10.1186/s12964-019-0366-y
%X Ori inhibited cytotoxicity and ROS generation in RAW 264.7 cells. a The chemical structure of Oridonin (Ori). b Cells were exposed to Ori (2.5, 5 or 10£¿¦ÌM) for 1£¿h and then trea ted with or without hydrogen peroxide (300£¿¦ÌM) for another 18£¿h. Cell viability was determined by MTT assay. c Cells were treated with or without Ori for 18£¿h, stained with 50£¿¦ÌM of DCFH-DA for 30£¿min, and subsequently exposed to hydrogen peroxide (300£¿¦ÌM) for 10£¿min to induce ROS generation. DCF fluorescence intensities were detected by a fluorescence microscope. d and e According to the related instructions, LAL enzyme and LPS-LBP-binding assay were measured by the different absorbance. f and g Cells were exposed to Ori (2.5, 5 or 10£¿¦ÌM) for 1£¿h, treated with or without LPS (1£¿¦Ìg/ml) for another 18£¿h and stained with 50£¿¦ÌM of DCFH-DA for 30£¿min. DCF fluorescence intensities were detected by flow cytometry. All results were expressed as the means ¡À SEM of three independent experiments. *p£¿<£¿0.05 and **p£¿<£¿0.01 versus the control group, #p£¿<£¿0.05 and ##p£¿<£¿0.01 versus LPS grou
%K Oridonin
%K Oxidative stress
%K Inflammation
%K Acute lung injury
%K Akt/Nrf2
%K MAPK/Nrf2
%K NOD-like receptor protein 3
%K NF-¦ÊB
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558832/