%0 Journal Article
%T Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens
%A Bao-Hua Zhang
%A Bo Zhai
%A Chang-Cheng Liu
%A Charles Ashton
%A Dan Tang
%A Gong-Bo Fu
%A Han Wu
%A He-Xin Yan
%A Hong-Dan Zhang
%A Hong-Ping Wu
%A Hong-Yang Wang
%A Jun Weng
%A Min Ding
%A Min Zeng
%A Wei-Feng Yu
%A Wei-Jian Huang
%A Xu Zhou
%A Yi Gao
%A Yong-Chao Cai
%A Zhi-Ying He
%J Archive of "Cell Research".
%D 2019
%R 10.1038/s41422-018-0103-x
%X Generation of human hepatocytes-derived liver progenitor-like cells in vitro. a Overview of the protocol used to convert PHCs into HepLPCs. b Light microscopy images of PHCs cultured in HGM or TEM at day 0 and day 10. Scale bars, 200£¿¦Ìm. c QPCR analyses for the expression of the indicated genes during TEM culture from day 0 to day 10. FLCs, fetal liver cells, 5 gestational weeks (one-way ANOVA with Dunnett correction for multiple comparisons, n£¿=£¿4 donors, *P£¿<£¿0.05, **P£¿<£¿0.01, ***P£¿<£¿0.001). d Immunofluorescence analyses demonstrating the expression of ALB and CK19. Scale bars, 50£¿¦Ìm. e Flow cytometric analysis showing the proportion of ALB or CK19 positive-cells in the populations. Blue, positive-cells; red, negative controls. f Growth curves illustrate the number of cells counted per well at different time points. Results expressed as mean£¿¡À£¿s.d. of three independent cultures derived from three donors. g Doubling time calculated for three donors. Error bars represent s.d.; n£¿=£¿3. h Edu incorporation is detected at passage 10. Scale bar, 50£¿¦Ìm. i Representative karyotype images of three independently established HepLPCs. Two lines maintained normal diploid karyotypes, whereas the third was partly triploid for Chromosome
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318298/