%0 Journal Article
%T A single circular chromosome yeast
%A Chen Cai
%A Fan Zhou
%A Guoping Zhao
%A Jin-Qiu Zhou
%A Ning Lu
%A Shanshan Wang
%A Xiaoli Xue
%A Yangyang Shao
%A Zhihu Zhao
%A Zhongjun Qin
%J Archive of "Cell Research".
%D 2019
%R 10.1038/s41422-018-0110-y
%X Characterization of the single circular chromosome yeast SY15. a Construction of the SY15 strain. Ligation of two chromosome ends via both CRISPR-Cas9 induced DSBs and homologous recombination. The red arrowheads indicated the cutting sites of Cas9. DR: direct repeat. The URA3 selection marker was further deleted via homologous recombination of two DR regions by negative selection. b The myc-tagged telomere binding protein Sir2 was detected with polyclonal anti-myc antibody and Cy3-conjugated (red) secondary antibody. Nop1, a nucleolar protein, was detected with a monoclonal anti-Nop1 antibody and Alexa 488-conjugated (green) secondary antibody. DNA was stained by DAPI (blue).1 c 3D conformation of the SY15 genome in comparison to that of SY14. d Classification of differentially expressed genes, defined as those with log2 (fold change) ¡Ý1 and P<0.05 in SY15 compared to SY14. Data were collected from three biological replicates. e Scanning electron microscopy pictures of SY14 and SY15 cells. Representative images from three independent experiments. f Heatmap of the Phenotype Microarray profiles of SY14 and SY15 cells. Low to high metabolic activities are depicted by a color spectrum from light blue to yellow. Data were collected from two biological replicates. g Cell cycle analysis. The yeast cells were synchronized with hydroxyurea and the progression of the cell cycle was analyzed by flow cytometry. Data are representative of two independent experiments. h Fitness analysis of SY15 cells under various growth conditions. Representative results of three independent experiments. i Senescence assay in liquid medium. The growth of wild type BY4742 (dark brown), SY14 (dark green), SY15 (dark blue), BY4742 tlc1¦¤ (light brown), SY14 tlc1¦¤ (light green), SY15 tlc1¦¤ (light blue) strains were monitored for 16 days. Every 24£¿h, the growth of the strains was measured in the value of OD600. The diluted cultures were started from OD600£¿=£¿0.01. For each strain, two clones were examine
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318310/