%0 Journal Article
%T Systematic epistatic mapping of cellular processes
%A Maximilian Billmann
%A Michael Boutros
%J Archive of "Cell Division".
%D 2017
%R 10.1186/s13008-016-0028-z
%X Genetic screens have identified many novel components of various biological processes, such as components required for cell cycle and cell division. While forward genetic screens typically generate unstructured ¡®hit¡¯ lists, genetic interaction mapping approaches can identify functional relations in a systematic fashion. Here, we discuss a recent study by our group demonstrating a two-step approach to first screen for regulators of the mitotic cell cycle, and subsequently guide hypothesis generation by using genetic interaction analysis. The screen used a high-content microscopy assay and automated image analysis to capture defects during mitotic progression and cytokinesis. Genetic interaction networks derived from process-specific features generate a snapshot of functional gene relations in those processes, which follow a temporal order during the cell cycle. This complements a recently published approach, which inferred directional genetic interactions reconstructing hierarchical relationships between genes across different phases during mitotic progression. In conclusion, this strategy leverages unbiased, genome-wide, yet highly sensitive and process-focused functional screening in cells
%K Genetic interactions
%K Image analysis
%K RNAi
%K Cell cycle
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223360/