%0 Journal Article
%T Ventral midbrain astrocytes display unique physiological features and sensitivity to dopamine D2 receptor signaling
%A Antonello Bonci
%A David Goldman
%A Kam-wing Jair
%A Kornel E. Schuebel
%A Lindsay M. De Biase
%A Raffaello Cimbro
%A Wendy Xin
%J Archive of "Neuropsychopharmacology".
%D 2019
%R 10.1038/s41386-018-0151-4
%X Morphology of ventral midbrain and telencephalic astrocytes. a Coronal brain section from a P60 Aldh1L1-eGFP mouse immunostained for Aldh1L1 (red). b Images taken from P60 Aldh1L1-eGFP mouse section showing density and tissue coverage by eGFP+ astrocyte processes in the ventral tegmental area (VTA), cortex (CTX), and hippocampus (HPC). c Closeup of individual eGFP+ astrocytes. d Quantification of eGFP+ astrocyte density in P60 animals. One-way ANOVA, F£¿=£¿0.6611, p£¿=£¿0.5441, N£¿=£¿5 animals, 1 section per animal, per region. e Quantification of percent tissue coverage by eGFP+ astrocyte processes in P60 animals (see Methods for details). One-way ANOVA, F£¿=£¿7.866, p£¿=£¿0.0025; Bonferroni post hoc tests for CTX vs HPC t£¿=£¿0.1441, CTX vs VTA t£¿=£¿3.536, HPC vs VTA t£¿=£¿3.396 (**p£¿<£¿0.01). N£¿=£¿8¨C9 sections, 4¨C5 animals per region. f Quantification of eGFP+ astrocyte soma size in P60 animals. One-way ANOVA, F£¿=£¿19.52, p£¿<£¿0.0001; Bonferroni post hoc tests for CTX vs HPC t£¿=£¿1.899, CTX vs VTA t£¿=£¿4.179, HPC vs VTA t£¿=£¿6.078 (***p£¿<£¿0.0001). N£¿=£¿6¨C8 sections, 4¨C6 animals per region. g Example scatter plot of one FACS experiment, with forward scatter values on the x-axis and FITC channel intensity on the y-axis. Circle indicates eGFP+ astrocyte considered in analysis. h Forward scatter values from one FACS experiment in which Aldh1L1-eGFP tissue microdissected from ventral midbrain, CTX, and HPC were dissociated into single-cell suspensions and analyzed by flow cytometry. Graph shows aggregate forward scatter values of eGFP+ astrocytes from the three regions. i Quantification of average forward scatter values (per animal/experiment) of eGFP+ astrocyte from ventral midbrain, CTX, and HPC. One-way ANOVA, F£¿=£¿31.06, p£¿<£¿0.0001; Bonferroni post hoc tests for CTX vs HPC t£¿=£¿0.5599, CTX vs ventral midbrain t£¿=£¿0.7089, HPC vs ventral midbrain t£¿=£¿6.529 (***p£¿<£¿0.001). N£¿=£¿6 animals/experiment
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300565/