%0 Journal Article
%T Whole-Brain Mapping of Direct Inputs to and Axonal Projections from GABAergic Neurons in the Parafacial Zone
%A Meng-Yang Gu
%A Xi Chu
%A Xiang Feng
%A Yan-Qin Yu
%A Yun-Ting Su
%J Archive of "Neuroscience Bulletin".
%D 2018
%R 10.1007/s12264-018-0216-8
%X Experimental procedures for retrograde monosynaptic tracing to identify direct inputs to PZ GABAergic neurons. A Design of viral vectors for RV-mediated trans-synaptic retrograde tracing. B Schematic of the PZ injection procedure for AAV-CAG-DIO-TVA-EGFP, AAV-CAG-DIO-RvG, and RV-EnvA-¦¤G-DsRed in VGAT-Cre mice. C Schematic showing unilateral injection site of virus in the PZ in VGAT-Cre mice. D Representative images showing RV-labeled neurons (red) in VGAT-Cre mice but not in wild-type mice (blue, nuclei stained with DAPI). Scale bar, 550 ¦Ìm. E Representative immunostaining images of the PZ from VGAT-Cre mice (left-most) showing that virus co-expression was restricted to the PZ. Scale bar, 200 ¦Ìm. Starter cells (yellow, expressing both EGFP and DsRed) indicated by arrows in enlarged view of the PZ (right). Scale bar, 20 ¦Ìm. 7n, facial nerve or its root
%K Parafacial zone
%K Parvocellular reticular formation
%K GABAergic neurons
%K Trans-synaptic tracing
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960452/