%0 Journal Article
%T Microbial culturomics to isolate halophilic bacteria from table salt: genome sequence and description of the moderately halophilic bacterium Bacillus salis sp. nov.
%A A. Diop
%A D. Raoult
%A E.H. Seck
%A J. Delerce
%A N. Armstrong
%A P.-E. Fournier
%A S. Khelaifia
%J Archive of "New Microbes and New Infections".
%D 2018
%R 10.1016/j.nmni.2017.12.006
%X Bacillus salis strain ES3T (= CSUR P1478 = DSM 100598) is the type strain of B. salis sp. nov. It is an aerobic, Gram-positive, moderately halophilic, motile and spore-forming bacterium. It was isolated from commercial table salt as part of a broad culturomics study aiming to maximize the culture conditions for the in-depth exploration of halophilic bacteria in salty food. Here we describe the phenotypic characteristics of this isolate, its complete genome sequence and annotation, together with a comparison with closely related bacteria. Phylogenetic analysis based on 16S rRNA gene sequences indicated 97.5% similarity with Bacillus aquimaris, the closest species. The 8 329 771 bp long genome (one chromosome, no plasmids) exhibits a G+C content of 39.19%. It is composed of 18 scaffolds with 29 contigs. Of the 8303 predicted genes, 8109 were protein-coding genes and 194 were RNAs. A total of 5778 genes (71.25%) were assigned a putative function
%K Bacillus salis
%K culturomics
%K genome
%K halophilic bacteria
%K human gut
%K taxonogenomics
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917939/