%0 Journal Article
%T Inhibition of HGF/MET signaling decreases overall tumor burden and blocks malignant conversion in Tpl2-related skin cancer
%A David Kowalski
%A Erik Maradiaga
%A J. Curtis Gwilliam
%A Kaitie Kudlac
%A Kathleen L. DeCicco-Skinner
%A Kira Flaherty
%A Lauren G. Falkenberg
%A Nicole F. Bonan
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-018-0109-8
%X a WST-1 viability assay for v-rasHa-transduced Tpl2£¿/£¿ and WT keratinocytes (RAS) grown in conditioned media from Tpl2£¿/£¿ or WT fibroblasts. Results normalized to the v-rasHa-transduced Tpl2+/+ (WT) keratinocyte control. *p£¿<£¿0.05, **p£¿<£¿0.01. b v-rasHa-transduced WT and Tpl2£¿/£¿ keratinocyte malignant conversion in response to fibroblast signaling. v-rasHa-transduced WT (¡°i¡±) or Tpl2£¿/£¿ (¡°ii¡±) keratinocytes cultured in high calcium media develop no foci and remain quiescent in a dispersed monolayer which can be seen at 4X (¡°iii¡±) and 40X (¡°iv¡±) magnification using rhodamine staining. WT (¡°v¡±) and Tpl2£¿/£¿ (¡°vi¡±) keratinocytes cultured in high calcium fibroblast conditioned media form proliferative foci. Foci from rhodamine-stained cultures stain brightly (¡°vi¡±, 4X magnification; ¡°vii¡±, 40¡Á magnification) under fluorescence microscopy. Real-time PCR quantification of HGF in keratinocytes (c) and immunoblotting (d) of HGF in untreated and v-rasHa-transduced Tpl2£¿/£¿ and WT keratinocytes. Immunoblotting of HGF in fibroblasts (e) and immunostaining of HGF in skin (f). Real-time PCR quantification (g) and immunoblotting (h) of p-MET. *p£¿<£¿0.0
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328619/