%0 Journal Article
%T A negative feedback loop between XBP1 and Fbw7 regulates cancer development
%A Ann- Hwee Lee
%A Bokyung Kim
%A Byeong Mo Kim
%A Dong-Seok Lee
%A Haiyoung Jung
%A Heejin Lee
%A Sang-Hyun Min
%A Unbin Chae
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-019-0124-4
%X a Co-immunoprecipitation (Co-IP) of transiently expressed XBP1s and FLAG-Fbw7. HEK293FT cells were co-transfected with XBP1s and FLAG-Fbw7. Immunoprecipitation was detected with anti-FLAG. b Co-immunoprecipitation (Co-IP) of transiently expressed XBP1s and FLAG-Fbw7. HEK293FT cells were co-transfected with XBP1s and FLAG-Fbw7. Immunoprecipitation was detected with anti-XBP1. c Immunoprecipitation of tunicamycin induced endogenous XBP1s and Fbw7. HEK293FT cells were treated 1£¿¦Ìg/ml tunicamycin 24£¿h. Immunoprecipitation was detected with anti-XBP1. d XBP1s interacts with Fbw7 in a phosphorylation-dependent manner. Transient expressing XBP1s and/or FLAG-Fbw7 lysates were treated with calf intestinal alkaline phosphatase (CIP), and subjected to co-immunoprecipitation. e Transient expressing XBP1 and/or FLAG-Fbw7 lysates were treated with ¦Ë-phosphatase, and subjected to co-immunoprecipitation. All western blot analyses were carried out with anti-XBP1and anti-FLAG antibodies. Actin was used for an internal contro
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381103/