%0 Journal Article
%T Single dose of DPX-rPA, an enhanced-delivery anthrax vaccine formulation, protects against a lethal Bacillusanthracis spore inhalation challenge
%A Bradley J. Berger
%A Genevieve M. Weir
%A Gloria S. Sivko
%A Jonathan Rayner
%A Leeladhar Sammatur
%A Lisa D. MacDonald
%A Marianne M. Stanford
%A Michelle W. Valderas
%A Rajkannan Rajagopalan
%J Archive of "NPJ Vaccines".
%D 2019
%R 10.1038/s41541-019-0102-z
%X Single-dose delivery of DPX-rPA (recombinant protective antigen) in mice induces rapid and sustained anti-rPA immunoglobulin G (IgG) response. Groups of nine CD-1 mice were vaccinated (intramuscularly (i.m.)) once with DPX-rPA dosed at either 10£¿¦Ìg (purple circles), 4£¿¦Ìg (gray squares), 2£¿¦Ìg (green diamonds), 0.5£¿¦Ìg (dark purple downwards triangles), or 0.05£¿¦Ìg (orange upwards triangles) or once with 10£¿¦Ìg alum-rPA control vaccine (black x). Immunizations are indicated with arrows. Endpoint anti-rPA IgG titers, presented as Log10 values, at Weeks 3, 4, 8, 12, 16, and 20 post immunization determined by enzyme-linked immunosorbent assay (ELISA) using plates coated with rPA antigen. Results are shown as mean£¿¡À£¿SEM. Significance shown was determined by two-way analysis of variance (ANOVA) with Tukey¡¯s multiple comparisons test of at least p£¿<£¿0.05, on Weeks 3, 4, 8 and 20: *, DPX-rPA (0.05£¿¦Ìg) compared to DPX-rPA (2, 4, 10£¿¦Ìg), DPX-rPA (0.5£¿¦Ìg) vs. DPX-rPA (4, 10£¿¦Ìg); +, DPX-rPA (0.05£¿¦Ìg) vs. DPX-rPA (2, 4, 10£¿¦Ìg), DPX-rPA (0.5£¿¦Ìg) vs. DPX-rPA (2, 4, 10£¿¦Ìg); £¿, DPX-rPA (0.05£¿¦Ìg) vs. DPX-rPA (2, 4, 10£¿¦Ìg), and alum, DPX-rPA (0.5£¿¦Ìg) vs. DPX-rPA (2, 4, 10£¿¦Ìg) and alum, DPX-rPA (2£¿¦Ìg) vs. DPX-rPA (10£¿¦Ìg), DPX-rPA (4£¿¦Ìg) vs. DPX-rPA (10£¿¦Ìg), DPX-rPA (10£¿¦Ìg) vs. alum; ¦Õ, DPX-rPA (0.05£¿¦Ìg) vs. all other groups, DPX-rPA (0.5£¿¦Ìg) vs. DPX-rPA (¦Ìg), DPX-rPA (2£¿¦Ìg) vs. DPX-rPA (10£¿¦Ìg), DPX-rPA (4£¿¦Ìg) vs. DPX-rPA (10£¿¦Ìg), DPX-rPA (10£¿¦Ìg) vs. alu
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368554/