%0 Journal Article
%T Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages
%A Alexander I. Makarkov
%A Angelica A. Gopal
%A Brian J. Ward
%A Elizabeth Ruiz-Lancheros
%A Ian N. Costas-Cancelas
%A Isabelle Rouiller
%A Makan Golizeh
%A Momar Ndao
%A Nathalie Charland
%A Nathalie Landry
%A Paul W. Wiseman
%A Sabrina Chierzi
%A Stephane Pillet
%J Archive of "NPJ Vaccines".
%D 2019
%R 10.1038/s41541-019-0111-y
%X Characterization of influenza HA presented on H1-VLPs and recombinant soluble H1 protein. a SDS-PAGE of H1-VLPs and soluble H1 protein (2.5 and 5.0£¿¦Ìg per sample) followed by Coomassie blue staining. Representative image from 3 experiments shown. b Immunoblot analysis of H1-VLPs and soluble H1 protein using rabbit polyclonal anti-H1 antibody. Representative image from 3 experiments shown. c Immunoblot analysis of H1-VLPs and soluble H1 protein using mouse monoclonal anti-H1 antibody. Representative image from 3 experiments shown. d Representative TEM image shows H1-VLPs with nanogold immunolabeled HA. Arrows indicate HA-bearing VLPs surrounded by nanogold particles on the surface of MDM exposed to H1-VLPs. Scale bar¨C100£¿nm. e Representative TEM image shows endosomal accumulation of nanogold immunolabeled HA in MDM. Arrows indicate HA-loaded endosomes in MDM exposed to H1-VLPs for 15£¿min. Scale bar¨C500£¿n
%K Recombinant vaccine
%K Cellular immunity
%K Influenza virus
%K Vaccines
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520342/