%0 Journal Article
%T Phosphorylation of 14-3-3¦Æ links YAP transcriptional activation to hypoxic glycolysis for tumorigenesis
%A Hui-Yan Li
%A Jue Wang
%A Ning Ma
%A Peng Zhang
%A Shi-Jing Mo
%A Ying Wang
%A Yu Jia
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-019-0143-1
%X a Subcellular fractionation analyses examining abundance of nuclear and cytoplasmic YAP protein expression in SW-1990 PDAC cells stimulated with hypoxia in the indicated times. GAPDH and Lamin B were used as internal control of cytoplasmic and nuclear extractions, respectively. SF serum-free, N normoxia, H hypoxia, WB western-blotting, NE nuclear extraction, CE cytoplasmic extraction, WCE whole cell extraction. Data are expressed as mean£¿¡À£¿s.d. of three independent experiments. *P£¿<£¿0.05. Two-sided Student¡¯s t test was used to calculate the P value. b Representative immunfluorescence images of nuclear YAP localization in SW-1990 PDAC cells stimulated with hypoxia in the presence or absence of Flag-tagged wild-type 14-3-3¦Æ transfection for 6£¿h. Scale bar£¿=£¿25£¿¦Ìm. c Subcellular fractionation analyses detecting abundance of nuclear and cytoplasmic YAP protein expression in hypoxia-stimulated SW-1990 PDAC cells with or without Flag-tagged wild-type 14-3-3¦Æ transfection. d Coimmunoprecipitation assay evaluating the interaction between 14-3-3¦Æ and YAP in SW-1990 PDAC cells stimulated with hypoxia for 6£¿h. Data are expressed as mean£¿¡À£¿s.d. of three independent experiments. *P£¿<£¿0.05. Two-sided ANOVA with Bonferroni post hoc t test correction was used to calculate the P valu
%K Cancer metabolism
%K Phosphorylation
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6510816/