%0 Journal Article
%T Identification by high-throughput sequencing of HPV variants and quasispecies that are untypeable by linear reverse blotting assay in cervical specimens
%A Alexis Proust
%A Claire Deback
%A Delphine Girlich
%A Fran£¿oise Bachelerie
%A J¨¦r£¿me Bouligand
%A Lucie Molet
%A R¨¦my A. Bonnin
%A S¨¦bastien Hantz
%J Archive of "Papillomavirus Research".
%D 2019
%R 10.1016/j.pvr.2019.100169
%X The linear reverse blotting assays are valid methods for accurate human papillomavirus (HPV) typing required to manage women at risk of developing cervical cancer. However, some samples showed a positive signal in HPV lines but failed to display a positive signal in subsequent typing lines (designated as HPV-X), which indicate that certain types were not available on the respective typing blots. The aim of this study is to elucidate the types or variants of HPV through the high-throughput sequencing (HTS) of 54 ASCUS cervical samples in which the viruses remained untypeable with INNO LiPA HPV£¿ assays. Low-risk (LR)-HPV types (HPV6, 30, 42, 62, 67, 72, 74, 81, 83, 84, 87, 89, 90 and 114), high-risk (HR)-HPV35 and possibly (p)HR-HPV73 were detected among HPV-X. Individual multiple infections (two to seven types) were detected in 40.7% of samples. Twenty-two specimens contained variants characterised by 2¨C10 changes. HPV30 reached the maximal number of 17 variants with relative abundance inferior or equal to 2.7%. The presence of L1 quasispecies explains why linear reverse blotting assays fail when variants compete or do not match the specific probes. Further studies are needed to measure the LR-HPV quasispecies dynamics and its role during persistent infection
%K Human papillomavirus
%K Variants
%K Genotyping
%K High-throughput sequencing
%K Quasispecies
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620621/