%0 Journal Article
%T Recombinant measles vaccine expressing malaria antigens induces long-term memory and protection in mice
%A Chantal Combredet
%A Chetan E. Chitnis
%A Claude Ruffi¨¦
%A Eduardo Aliprandini
%A Fr¨¦d¨¦ric Tangy
%A Marie Mura
%A Pauline Formaglio
%A Rogerio Amino
%J Archive of "NPJ Vaccines".
%D 2019
%R 10.1038/s41541-019-0106-8
%X rMV-CSPb and rMV CSPf design, rescue and characterization. a Schematic representation of measles vector expressing CS protein from Plasmodium berghei ANKA (CSPb) and Plasmodium falciparum (CSPf). The synthetic sequences were mammalian codon-optimized and cloned into the additional transcription unit (ATU) position 2 of pTM-Schwarz. The MV genes are indicated as follows: nucleoprotein (N), phosphoprotein (P), V and C accessory proteins, matrix (M), fusion (F), hemagglutinin (H) and polymerase (L). T7 RNA polymerase promoter (T7), T7 RNA polymerase terminator (T7t), hepatitis delta virus ribozyme (£¿), hammerhead ribozyme (hh) are requested for viral rescue. b Growth curves of MV-Schwarz, rMV-CSPb, and rMV-CSPf in Vero cells infected at an MOI of 0.1. Cell-associated virus titers are indicated in TCID50/ml. c Detection by western-blot of CSPb and CSPf in cell lysates (L) or supernatant (SN) of Vero cells infected by rMV-CSPb and rMV CSPf. Blots derived from the same experiment and were processed in parallel. d Immunofluorescence detection of CSPb and CSPf in Vero cells infected for 24£¿h with rMV-CSPb and rMV-CSPf at an MOI of 0.1. Infected cells formed syncytia which are localized CS protein
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393439/