%0 Journal Article
%T Boosting BCG with proteins or rAd5 does not enhance protection against tuberculosis in rhesus macaques
%A Amy J. Myers
%A Charles A. Scanga
%A Dominick Laddy
%A Hannah P. Gideon
%A JoAnne L. Flynn
%A Joshua A. Hackney
%A Mario Roederer
%A Mark A. Rodgers
%A Patricia A. Darrah
%A Pauline Maiello
%A Peter Andersen
%A Philana Ling Lin
%A Robert A. Seder
%A Robert M. DiFazio
%A Thomas Evans
%A Thomas Lindenstrom
%A Victor Prikhodko
%J Archive of "NPJ Vaccines".
%D 2019
%R 10.1038/s41541-019-0113-9
%X Study design. A total of 64 rhesus macaques were enrolled into three challenge cohorts (A/B, n£¿=£¿20 each; C, n£¿=£¿24) and eight experimental groups: unvaccinated (No Vax, n£¿=£¿8 in Cohort A/B and n£¿=£¿4 in Cohort C presented separately because of differences in sampling schedules between cohorts); BCG-primed (BCG, n£¿=£¿8); BCG-primed and boosted IM/AE 20 or 25 weeks later with either an empty Ad5 vector [Ad5(Empty), n£¿=£¿7], Ad5 expressing ESAT-6 and Ag85B [Ad5(EB), n£¿=£¿8]; Ad5 expressing M72 [Ad5(M72), n£¿=£¿8]; or Ad5 expressing ESAT-6, Rv1733, Rv2626, and RpfD [Ad5(4Ag), n£¿=£¿7]; BCG-primed and boosted IM twice (4 or 5 weeks apart) 16¨C25 weeks later with either M72 protein or H56 protein in adjuvant (M72/AS01E, n£¿=£¿8 or H56:CAF01, n£¿=£¿6). Three months after boosting (32¨C38 weeks after BCG), macaques were challenged with a low dose of Mtb Erdman (8¨C16 CFU) and monitored for 6 months post challenge (study endpoint
%K Immunology
%K Microbiology
%K Diseases
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6538611/