%0 Journal Article
%T Clostridium perfringens epsilon toxin vaccine candidate lacking toxicity to cells expressing myelin and lymphocyte protein
%A Brendan W. Wren
%A Helen Morcrette
%A Leo Bennett
%A Monika Bokori-Brown
%A Nick Lewis
%A Richard W. Titball
%A Stephanie Ong
%J Archive of "NPJ Vaccines".
%D 2019
%R 10.1038/s41541-019-0128-2
%X Variant proteins tested in this study. a The location of residues previously mutated (Y30 and Y196; ref. 20) are shown in red and the location of residues in the ¦Â-octyl-glucoside binding cleft (V72, F92, H149, V166, A168; ref. 19) mutated in this study are shown in green. Purified proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and visualised by Coomassie staining before b and after c activation with trypsin. Lane 1£¿=£¿molecular weight marker (kDa); 2£¿=£¿wild-type toxin; 3£¿=£¿Y30A-Y196A; 4£¿=£¿Y30A-Y196A-H149A; 5£¿=£¿Y30A-Y196A-A168F; 6£¿=£¿Y30A-Y196A-F92A; 7£¿=£¿Y30A-Y196A-V166A; 8£¿=£¿Y30A-Y196A-V72F; 9£¿=£¿Y30A-Y196A-H149A-A168F. Gels shown derive from the same experiment and were processed in paralle
%K Protein vaccines
%K Bacterial infection
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6667452/