%0 Journal Article
%T Recovery of stem cell proliferation by low intensity vibration under simulated microgravity requires LINC complex
%A A. N. Senthilnathan
%A G. Uzer
%A H. Touchstone
%A J. Alwood
%A J. Rubin
%A J. T. Oxford
%A K. Puranam
%A M. Thompson
%A R. Beard
%A R. Bryd
%A S. Kim
%A S. Loisate
%A X. Pu
%J Archive of "NPJ Microgravity".
%D 2019
%R 10.1038/s41526-019-0072-5
%X Simulated microgravity (sMG) decreases cell proliferation without affecting cell viability. a MSCs were subjected to simulated microgravity (sMG) via rotation at a constant 15 RPM for 3 days inside 12.5£¿cm2 sealed flasks. LIV was applied twice daily at 2£¿h intervals for 3 days outside the incubator at 0.7£¿g¡¯s and 90£¿Hz for 20£¿min. During LIV, all samples were taken out but only sMG£¿+£¿LIV group was vibrated. Samples were collected for analysis 24£¿h after last LIV treatment. b Comparing the cell proliferation and dead cell to live cell ratio using microscopy indicated less cells in field of view (10£¿¡Á), flow cytometry showed that sMG decreases cell proliferation by 2.63-fold (p£¿<£¿0.01, n£¿=£¿6/group). Cell death was not different between difference Ctrl and sMG samples (n£¿=£¿6/group). Group comparisons were made using unpaired T-test (b). *p£¿<£¿0.05, **p£¿<£¿0.01, ***p£¿<£¿0.001, against control and each othe
%K Stem cells
%K Biomedical engineering
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520402/