%0 Journal Article
%T CRISPR/Cas9 ¨C An evolving biological tool kit for cancer biology and oncology
%A Ann M. Bode
%A Mee-Hyun Lee
%A Satyananda Patel
%A Tingxuan Gu
%A Xueli Tian
%A Zigang Dong
%J Archive of "NPJ Precision Oncology".
%D 2019
%R 10.1038/s41698-019-0080-7
%X CRISPR/Cas9-based gene modification. Common methods of delivering the CRISPR system include a plasmid-based method and Cas9 protein complex with sgRNA or RNP. After the sgRNA binds to the target site of genomic DNA, the Cas9 protein creates a DSB around the PAM site. Random indels or precise modifications introduced into the genomic DNA by the NHEJ or HDR pathwa
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423228/