%0 Journal Article
%T Proteomic analysis of human mesenchymal stromal cell secretomes: a systematic comparison of the angiogenic potential
%A Anna Mallone
%A Anne-Christine Uldry
%A Benedikt Weber
%A Benjamin Gantenbein
%A Debora Kehl
%A Manfred Heller
%A Melanie Generali
%A Phil Cheng
%A Simon P. Hoerstrup
%J Archive of "NPJ Regenerative Medicine".
%D 2019
%R 10.1038/s41536-019-0070-y
%X Characterization of hMSC from adipose tissue, bone marrow and umbilical cord Wharton¡¯s jelly. a Trilineage differentiation of hMSCs (n£¿=£¿5 per hMSC tissue source) into adipogenic (left; Oil Red O staining), osteogenic (middle; Alzarin Red S) and chondrogenic (right; Alcian Blue PAS) lineages. Scale bar£¿=£¿100£¿¦Ìm. b Proliferation capacities of hMSCs (n£¿=£¿5 per hMSC tissue source) over 12 days, c showing the cumulative population doublings of each hMSC source. d All hMSCs (n£¿=£¿5 per hMSC tissue source) positively expressed CD73, CD90, CD105, and only a minimal proportion of cells were positive for CD14, CD34 and CD45. Data are visualized by bi-dimensional t-SNE maps generated from multidimensional flow cytometry data. e Recorded events were subdivided into 5 clusters by PhenoGraph and FlowSom algorithms according to surface marker expression. The cellular distribution of 5 donors per cell source is f visualized with cumulative density plots and g quantified accordingly. Bar graphs present mean£¿¡À£¿s.d. (*p£¿<£¿0.05, **p£¿<£¿0.01, ***p£¿<£¿0.001; one-way ANOVA and Tukey multiple comparison
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467904/