%0 Journal Article
%T Dihydroartemisinin inhibits prostate cancer via JARID2/miR-7/miR-34a-dependent downregulation of Axl
%A Durairaj Sekar
%A Juliano D. Paccez
%A Kelly Chibale
%A Kristal Duncan
%A Luiz F. Zerbini
%A Manoj Bashin
%A Ricardo G. Correa
%A Towia A. Libermann
%A Xuesong Gu
%A Yihong Wang
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-019-0122-6
%X a qRT-PCR for Axl in PCa cells treated with 5£¿¦ÌM of DHA for 24£¿h. Values were normalized to Gapdh levels and to DMSO control. The experiment was performed in triplicate. Data are representative of 3 independent experiments; *p£¿<£¿0.05 two-tailed Student¡¯s t-test. b Immunoblot analysis of protein extracts obtained from DU145 and PC-3 treated with 5£¿¦ÌM DHA for 24£¿h using anti-phospho-Axl, anti-phospho-Akt and anti-phospho-Stat3 and anti-Axl, anti-Akt, anti-Stat3 and anti- GAPDH. c Migration and d invasion analysis of mCRPC cell lines 24£¿h post treatment with 5£¿¦ÌM of DHA. Cells were fixed and stained, and 3¨C5 random microscopic fields were counted. Data are representative of three independent experiments and all values shown are mean£¿¡À£¿SD from a representative experiment; **p£¿<£¿0.01, two-tailed Student¡¯s t-test. e Apoptosis assay for mCRPCa cell lines treated with 5£¿¦ÌM of DHA for 8£¿h. DMSO 0.05% was used as a control. Data are representative of three independent experiments and all values shown are mean£¿¡À£¿SD from a representative experiment; *p£¿<£¿0.05, two-tailed Student¡¯s t-tes
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381097/