%0 Journal Article
%T RelB acts as a molecular switch driving chronic inflammation in glioblastoma multiforme
%A Angela S. Gupta
%A Debolina D. Biswas
%A Karli Mockenhaupt
%A LaShardai N. Brown
%A Michael R. Waters
%A Tomasz Kordula
%J Archive of "Oncogenesis".
%D 2019
%R 10.1038/s41389-019-0146-y
%X a Workflow of CytoAnalysis to establish inflammatory signature of GBM patients (TCGA, n£¿=£¿208). For each cytokine and cytokine receptor gene, the mean Z-score normalized expression values were used to group patients into high and low expressors. Kaplan¨CMeier analysis was performed yielding a p-value indicating the prognostic relationship between each gene and survival. These p-values were used to rank cytokines and cytokine receptors as indicators of poor prognosis. A combined prognostic score was then generated for each cytokine and its receptor. b Inflammatory signature (combined rank score) of GBM tumors determined by CytoAnalysis. c, d Kaplan¨CMeier analysis for individual cytokines and their receptors. Patients were annotated as high and low expressors using the mean Z-score expression level as a cutoff. Statistical significance was assessed using the Cox-proportional hazards model, *p£¿<£¿0.05. e Pearson correlation of IL-1¦Â and OSM mRNA expression scores for GBM patients (TCGA, n£¿=£¿208). Normalized Z-score expression values were downloaded and Pearson correlation was conducted using cBioPortal. Both p-value and regression analysis was performed using the ¡®lm¡¯ function in the core R statistical package. f Genome-wide gene rank of Pearson correlation with IL1B. Gene expression correlations were performed using cBioPortal. g Kaplan¨CMeier survival analysis of patients expressing either high or low levels of both IL-1¦Â and OSM, analyzed as in c. *p£¿<£¿0.05. h Patient subtype was downloaded using clinical expression information contained within TCGA (n£¿=£¿206). *p£¿<£¿0.05; One-way ANOV
%K CNS cancer
%K Inflammation
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541631/