%0 Journal Article
%T Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs
%A Justin Blonigan
%A Marco Quarta
%A Melinda J. Cromie Lear
%A Patrick Paine
%A Robert Chacon
%A Thomas A. Rando
%J Archive of "NPJ Regenerative Medicine".
%D 2018
%R 10.1038/s41536-018-0057-0
%X a Relationship between maximum isometric tetanic force measured ex vivo and muscle mass in mouse TAs. Control muscles are designated as ¡°VML£¿ Scaffold£¿ Cells£¿¡±. In the VML injury groups, muscles were partially excised and left untreated (VML+ Scaffold£¿ Cells£¿) or implanted with either a tissue-engineered bioconstruct comprising of scaffold alone (VML+ Scaffold+ Cells£¿) or with scaffold and muscle stem cells, and muscle-resident cells (VML+ Scaffold+ Cells+). ¡°VML+ Scaffold+ Cells+¡± muscles showed proportionally increased mass and, statistically significant, force (Table (Table1),1), consistent with functional active stress generation in the newly formed tissue. b Active twitch force across a range of muscle lengths. (Left panel) In vivo measurements. (Right panel) Ex vivo measurements. The ¡°VML+ Scaffold£¿ Cells£¿¡± muscles have narrowed length-tension curves (comparisons between VML+ Scaffold+ Cells£¿ group and VML+ Scaffold+ Cells+ or VML£¿ Scaffold£¿ Cells£¿ groups; p£¿<£¿0.0001). The length-tension curves of the ¡°VML+ Scaffold+ Cells+¡± muscles were restored with treatment, meaning that a greater fraction of the maximum force was generated over a broader range of muscle lengths. No improvement was observed with ¡°VML+ Scaffold+ Cells£¿¡± treatment. The curve from each muscle was normalized by its own maximum force and centered at optimal length. Symbols are the mean forces and error bars represent SEM (n£¿=£¿6¨C9 muscles per group
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180087/