%0 Journal Article
%T NDRG4 promoter hypermethylation is a mechanistic biomarker associated with metastatic progression in breast cancer patients
%A Alfredo Carlos S. D. de Barros
%A Ana Paula M. da Silva
%A Anamaria A. Camargo
%A Andre Carvalho
%A Camila L. Machado
%A Dirce M. Carraro
%A Elisa H. F. Jandrey
%A Fernando A. Soares
%A Helena P. Brentani
%A Isabela W. da Cunha
%A Katia Ramos M. Leite
%A Lilian T. Inoue
%A Luciana N. S. Andrade
%A Luiz Felipe Campesato
%A Paula F. Asprino
%A Raphael B. Parmigiani
%A Ricardo P. Moura
%A Roger Chammas
%A Vladmir C. de Lima
%A ¨¦rico T. Costa
%J Archive of "NPJ Breast Cancer".
%D 2019
%R 10.1038/s41523-019-0106-x
%X Methylation landscape and expression analysis of NDRG4 gene in breast tumor cell lines. a Schematic representation of NDRG4 genomic region, including promoter (positions £¿1500 to +500 from TSS), CpG island (£¿556 to +869), transcription start site (TSS) and first exon. A CpG island with 82 CGs located at positions £¿387 to +103 from TSS was represented in detail. b¨Cd Percentages of methylation in these positions in two breast tumor cell lines (MCF-7 and MDA-MB231) and one melanoma cell line (MDA-MB435) before (ctrl) and 6 days after 5-aza-dC treatments (1£¿¦ÌM). The percentage of DNA methylation (y-axis) is shown as average C/T ratios. Graphs represent mean percent DNA methylation at each CpG and its position relative to TSS (n£¿=£¿5). e Real-time PCR analysis of NDRG4 expression in MCF-7, MDA-MB231 and MDA-MB435 before (control) and 6 days after 5-aza-dC treatments. Expression levels relative to MCF-7 control cells and normalized to GAPDH endogenous control. Error bars represent SEM of biological replicates (n£¿=£¿3). p£¿<£¿0.05 by two-tailed t tes
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450950/