%0 Journal Article
%T Transcriptional regulation of Bcl-2 gene by the PR/SET domain family member PRDM10
%A Jieying Gao
%A Na Chen
%A Shi Huang
%A Taobo Hu
%A Yuanyuan Gui
%A Zhihong Li
%J Archive of "PeerJ".
%D 2019
%R 10.7717/peerj.6941
%X Bcl-2 (B-cell lymphoma 2) protein is localized in the outer membrane of mitochondria, where it plays an important role in promoting cellular survival and inhibiting the actions of pro-apoptotic proteins. PRDM10 is a member of the PR/SET family of epigenetic regulators and may play a role in development and cell differentiation. Here we show that human PRDM10 contributes to the transcriptional regulation of human Bcl-2 gene. We found that PRDM10-depletion in human cells reduced the expression of Bcl-2 protein and over-expression of PRDM10 promoted Bcl-2 protein expression. Furthermore, luciferase reporter activity of Bcl-2 gene P1 promoter was significantly increased in cells co-transfected with PRDM10, and PRDM10 was able to bind to the Bcl-2 P1 promoter in vivo. Using The Cancer Genome Atlas (TCGA) data set, we found weak positive correlation between PRDM10 and Bcl-2 in several cancer types including cancers of the breast, colon, and lung tissues. These data identify a novel function for PRDM10 protein and provide insights on the transcriptional control of Bcl-2 expression
%K PRDM10 protein
%K Bcl-2
%K B-cell lymphoma-2
%K Transcription
%K The Cancer Genome Atlas
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525587/