%0 Journal Article
%T A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST
%A Barry N. Kreiswirth
%A Dror Marchaim
%A Dubert M. Guerrero
%A Federico Perez
%A Keith S. Kaye
%A Latania K. Logan
%A Lee M. Kiedrowski
%A Maria Virginia Villegas
%A Mark Adams
%A Robert A. Bonomo
%A Roberto A. Viau
%J Archive of "Pathogens and Immunity".
%D 2017
%R 10.20411/pai.v2i1.99
%X Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST). In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation
%K bacterial typing
%K molecular epidemiology
%K Enterobacter cloacae
%K infectious diseases outbreaks
%K rep-PCR
%K MLST
%K hsp60
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394936/